Regulatory
yenbox_J23

Part:BBa_K1381001:Design

Designed by: Stephanie Herman, Gunta Celma, Megha Biradar, Christoffer Andersson and Martin Friberg   Group: iGEM14_Uppsala   (2014-10-02)

yenbox_J23113


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 41
    Illegal NheI site found at 52
    Illegal NheI site found at 75
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The source of the yenbox sequence in this part is the genome of Yersinia enterocolitica and the source of the promoter is from the BioBrick BBa_J23113 (J23113).

Source

The sequence of the yenbox was taken from Y. enterocoliticas genome and the promoter sequence of the promoter was taken from the Anderson promoter BBa_J23113 (J23113).

References

Ching-Sung Tsai and Stephen C. Winanas, The quorum-hindered transcription factor YenR of Yersinia enterocolitica inhibits pheromone production and promotes motility via a small non-coding RNA, 2011, Molecular Microbiology 80[2], 556-571